2. A restriction enzyme is added to the DNA to cut it into fragments
3. DNA fragments are separated according to size during a process called electrophoresis using an agarose gel
4. An alkali is added to separate the double stranded DNA into single strands
5. Each single strand is transferred to a nylon membrane and a radioactive probe is added to it
6. A banding pattern appears
7. An x-ray film is produced and the positions of black bands are compared with the part of DNA treated with radioactive probe
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